CycloneSEQ

CycloneSEQ Technical Principle

CycloneSEQ™ is a nanopore sequencing technology platform officially launched by the BGI Group in 2024. During the sequencing process of CycloneSEQ, the DNA library molecules linked to the motor proteins approach the entrance of the nanopore protein under the influence of an electric field. When the motor proteins reach the nanopore entrance, they are activated, effectively and rapidly unwinding the DNA, allowing the library to pass through the nanopore protein as single-stranded molecules. As different DNA bases and their arrangements obstruct the current to varying degrees, fluctuations in the current occur as the DNA passes through the nanopore. The channel sensors on the sequencing flow cell capture these current signals, which are then analyzed by the control software to obtain real-time information about the base sequence of the sample being tested.

Flow CellThe device for nanopore sequencing reactions and electrical signal detection.

Motor ProteinSeparates double-stranded DNA into single strands and mediates the passage of single-stranded nucleic acids through nanopore proteins.

Nanopore ProteinImmobilized on the membrane to function as channels for ions or nucleic acid molecules.

MembraneEmbedded with nanopore proteins, stabilizes sequencing reactions and prevents interference from macromolecular signals.

Channel SensorThe core electronic component of nanopore sequencing, responsible for processing electrical

Software & AlgorithmThe software controls the sequencing reactions, while the algorithm model converts raw electrical signals into base sequence information.

Key Technical Advantages

Explore the Advantages of Nanopore Technology

Full-length readsNanopore sequencing can capture and analyze DNA molecules ranging from short fragments to ultra-long reads, providing uninterrupted, full-length sequence information.

Direct sequencingNanopore sequencing enables direct sequencing of unmodified or unamplified nucleic acid molecules. This preserves original genetic information and offers potential for multi-omics expansion.

Real-time detectionNanopore sequencing supports real-time data analysis, significantly reducing the turnaround time from sample collection to result interpretation.

PortabilityThe compact size of the sequencers makes them easily portable and greatly extends the use of on-site sequencing.

Simple and rapidThe experiment process is streamlined to enable rapid completion of the entire process, from sample preparation to final data analysis.